The DNA/RNA Delivery Core is directed by Irina V. Budunova, MD, PhD, and co-directed by Associate Core Director, Alexander Y. Yemelyanov, MD, PhD with Advisor Eric L. Weiss, PhD. The major objective of the DNA/RNA Delivery Core is to support SDRC researchers and P&F projects through the provision of innovative viral technologies for in vitro and in vivo applications, including the expansion of expression vector and viral stock libraries, and individual training for users.
- Lentiviral/retroviral vector systems through lentiviral vector library and generation of custom vectors.
- Lentiviruses and Retroviruses provided in two formats: standard titer: 107-108 TU/mL high titer: 109-1010 TU/mL at customized amounts.
- shRNA/MiR gene silencing using NU mouse/human whole genome shRNA lentiviral library and tested commercial libraries (Dharmacon, Sigma, Origene, Genecopaeia).
- CRISPR-mediated genome editing including ORF change, deletion of gene region, and mutagenesis. The service provides 3-4 single cell clones (guaranteed 100% homogeneous clones) for 2 independent gRNAs.
- CRISPR-epigenetic regulation of gene expression (requires customer’s consultation to start the project).
- Stably infected cell cultures including primary and immortalized skin human and mouse cells (keratinocytes, fibroblasts, melanocytes and neurons) and other cells provided by customers.
- Transient Transfections of Cells by Electroporation utilizing the LONZA device
- Targeted single cell delivery (proteins, viruses, fluorescent probes, DNA/RNA, drugs etc) using NFPE, Nanofountain Probe Electroporation (contact for experimental design and consultation to start the project).
Irina V. Budunova, MD, PhD
Associate Professor of Dermatology and Urology
Alexander Y. Yemelyanov, MD, PhD
Associate Core Director
Research Assistant Professor of Medicine, Pulmonary Division
We welcome your interest in our core. Please contact Research Associate Pankaj Bhalla, PhD, for more information.
Pankaj Bhalla, PhD